منابع مشابه
Methods for culturing mouse and human embryonic stem cells.
Mouse embryonic stem cells (mESCs) were first derived and cultured almost 30 years ago and ever since have been valuable tools for creating knockout mice and for studying early mammalian development. More recently (1998), human embryonic stem cells (hESCs) have been derived from blastocysts, and numerous methods have evolved to culture hESCs in vitro in both complex and defined media. hESCs are...
متن کاملCulturing Uveal Melanoma Cells.
A major challenge in cancer research is the use of appropriate models with which to study a specific biological question. Cell lines have long been used to study cellular processes and the effects of individual molecules because they are easy to use, grow rapidly, produce reproducible results and have a strong track record in research. In uveal melanoma in particular, the absence of animal mode...
متن کاملPreparation of Mouse Embryonic Fibroblast Cells Suitable for Culturing Human Embryonic and Induced Pluripotent Stem Cells
In general, human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs)(1) can be cultured under variable conditions. However, it is not easy to establish an effective system for culturing these cells. Since the culture conditions can influence gene expression that confers pluripotency in hESCs and hiPSCs, the optimization and standardization of the culture method is cr...
متن کاملNeural induction in mouse embryonic stem cells by co-culturing with chicken somites.
The role of paraxial mesoderm or the somites in decision of ectoderm to acquire the neuroepithelial fate and its subsequent diversification to functional neural subtypes especially in mammalians is obscure. Here we report, for the first time, the influence of the co-culture of alginate bead-encapsulated somites isolated from chick embryos on neural differentiation in mouse embryonic stem cells-...
متن کاملNovel method for culturing Schwann cells from adult mouse sciatic nerve in vitro.
Schwann cells (SCs) are important in the recovery of peripheral nerve injury and are valuable cells for the tissue engineering of artificial neurons. Clinical applications that require pure SCs in large quantities are limited since human and mouse SCs do not attach well to the wall of the culture dish and have low proliferative potential. To obtain high quantities of highly pure SCs, we develop...
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ژورنال
عنوان ژورنال: Cold Spring Harbor Protocols
سال: 2015
ISSN: 1940-3402,1559-6095
DOI: 10.1101/pdb.top069989